產(chǎn)品介紹 品名 Monoclonal Antibody to Human Factor B (Bb) 貨號 A227 規(guī)格 100 μL 同種型 IgG1K 蛋白質濃度 > 1.0 mg/mL 純度 ≥ 95% by SDS PAGE 緩沖液 Borate Buffered Saline (pH 8.4 ± 0.2) 物種交叉反應 None tested. 儲存 ≤ 30 Days 2-8?C > 30 Days ≤ –20?C 使用說明 For Research Use Only. Not for use in Diagnostic Procedures 背景 Factor B is one of the complement proteins which is unique to the Alternative Pathway. It is a single-chain glycoprotein with a molecular weight of approximately 98 kD. The concentration of Factor B in human serum/plasma is ~200 μg/ml. The Alternative Complement Pathway provides innate protection against microbial agents in the absence of specific antibodies. The activation of this complement pathway can be triggered by a variety of substances, including microbial polysaccharaides or lipids, gram-negative bacterial lipopolysaccharides, and surface determinants present on some viruses, parasites, virally infected mammalian cells, and cancer cells. In autoimmune diseases, the Alternative Complement Pathway may contribute directly to tissue damage. 特異性 The specificity of the monoclonal antibody for the Factor B (Bb) protein was established by ELISA. The antibody was shown to bind highly purified Factor B and its Bb fragment, but not to the Ba fragment. The antibody binds a non-neoantigen in the Bb domain of native Factor B, since highly purified, functionally active Factor B and normal human plasma blocked binding of the antibody to both Factor B and Bb fragments coated in microtiter wells. Zymosan activated human serum was not more effective than the corresponding non-activated plasma in blocking the binding of this antibody. 應用 參考資料 1、Wright, A., Morrison, S. Effect of C2 Associated Carbohydrate structure of Ig Effector function: studies with chimeric mouse human IgG1 Antibodies in glycosylation mutants of chinese hamster ovary cells, J. Immunol 160:3393-3402, 1998. 2、Hourcade, D., et al. A conserved element in the serine protease domain of complement Factor B, J Bio Chem 273:25996-26000, 1998.
產(chǎn)品介紹
Monoclonal Antibody to Human Factor B (Bb)
A227
100 μL
IgG1K
> 1.0 mg/mL
≥ 95% by SDS PAGE
Borate Buffered Saline (pH 8.4 ± 0.2)
None tested.
≤ 30 Days 2-8?C
> 30 Days ≤ –20?C
For Research Use Only. Not for use in Diagnostic Procedures
Factor B is one of the complement proteins which is unique to the Alternative Pathway. It is a single-chain glycoprotein with a molecular weight of approximately 98 kD. The concentration of Factor B in human serum/plasma is ~200 μg/ml.
The Alternative Complement Pathway provides innate protection against microbial agents in the absence of specific antibodies. The activation of this complement pathway can be triggered by a variety of substances, including microbial polysaccharaides or lipids, gram-negative bacterial lipopolysaccharides, and surface determinants present on some viruses, parasites, virally infected mammalian cells, and cancer cells. In autoimmune diseases, the Alternative Complement Pathway may contribute directly to tissue damage.
The specificity of the monoclonal antibody for the Factor B (Bb) protein was established by ELISA. The antibody was shown to bind highly purified Factor B and its Bb fragment, but not to the Ba fragment. The antibody binds a non-neoantigen in the Bb domain of native Factor B, since highly purified, functionally active Factor B and normal human plasma blocked binding of the antibody to both Factor B and Bb fragments coated in microtiter wells. Zymosan activated human serum was not more effective than the corresponding non-activated plasma in blocking the binding of this antibody.
1、Wright, A., Morrison, S. Effect of C2 Associated Carbohydrate structure of Ig Effector function: studies with chimeric mouse human IgG1 Antibodies in glycosylation mutants of chinese hamster ovary cells, J. Immunol 160:3393-3402, 1998.
2、Hourcade, D., et al. A conserved element in the serine protease domain of complement Factor B, J Bio Chem 273:25996-26000, 1998.
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