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 Borate Buffered Saline

 Baboon, Cynomolgus Monkey

 2°C to 8°C* (≤ 30 days)

For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw

 For Research Use Only. Not for use in diagnostic procedures

 All of Quidel’s monoclonal antibodies to complement antigens were prepared using intact complement proteins and are purified from mouse ascites fluid via protein A affinity chromatography. The prepared monoclonal antibodies are buffer exchanged in Borate Buffered Saline containing 0.02% NaN3

Factor H is a fluid phase complement regulatory protein consisting of a single peptide chain of 20 short consensus repeat segments or CCP’s with a molecular weight of approximately 155 KD. 1 Factor H regulates the alternative pathway of the complement system by modifying activity of the “feedback loop.” It does this in three ways. First, it is a co-factor for the serine protease Factor I, which cleaves C3b to iC3b. iC3b has no hemolytic or amplification function, but may be bound by complement receptors. Second, Factor H prevents the formation of and accelerates the disassociation of the alternative pathway C3 convertase, C3bBb from cell surfaces. Finally, Factor H binds to polyanions on host cell surfaces and tissue matrices, such as basement membranes, blocking deposition of C3b. This later activity is leveraged by many pathogens as a mode of complement evasion. 2

Applications of this antibody have been evaluated by various research facilities, and include EIA, 5 Western Blot, 6 and FACS

1、Pangburn M.K. Differences between the binding sites of the complement regulatory proteins DAF, CR1 and Factor H on C3 Convertases, J Immunol 136:6, 1986.

2、Kraiczy P, Würrzner, R. Complement escape of human pathogenic bacteria by acquisition of complement regulators, Mol Immunol 43:31-44, 2006.

3、Atkinson, J.P., et al. Complement factor H and the hemolytic uremic syndrome, JEM 204:6, 1245-1248, 2007.

4、Sivaprasad, S. and Chong, N.V. The Complement system and age-related macular degeneration, Eye 1-6, 200