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20.0 kDa; due to varying degrees of glycosylation, migrates to 25-37kDa on Western Blot

Recombinant DNA expressed in human cells

Greater than 95% as determined by ion exchange, hydrophobic interaction and/or size exclusion chromatography
Endotoxin levels < 1 EU/ug

Measured in cytopathic effect inhibition assay on human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV), EC50 ~1 U/ml

Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

< 1 EU/μg

Ion exchange, hydrophobic interaction and/or size exclusion chromatography

For retention of full activity store at -70 oC or below and avoid repeated freeze-thaw cycles