This broad spectrum Protease Inhibitor cocktail has been specifically designed to provide complete protection of valuable proteins of interest from degradation during extraction and purification.
Features and Benefits: Complete protection from proteases in one single cocktail.
All our cocktails contain AEBSF, the preferred serine protease inhibitor, to provide superior protection with unmatched stability, convenience and reliability.
The cocktail formulations are available with or without EDTA which may interfere with protein stability or certain downstream applications.
Our cocktails are lyophilized solids which dissolve instantaneously making them readily available for their instantaneous inhibitory action and are easier to use than tablets that require splitting, vortexing and time consuming dissolution.
Our lyophilized solids are guaranteed for stability, reproducibility and have a shelf life of over a year. Most other commercially available cocktails made and sold in aqueous solutions offer very low guarantee of stability after 1 month of being manufactured (Lit ref: Bestatin, Leupeptin solution in water are stable for 1 month at -20oC).
Economical: More cost effective than other commercially available formulations.
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Reliable: Count on our expertise in the production of high quality cocktails that have been successfully used for 20 + years by thousands of researchers worldwide.
Product Description: White Lyophilized Solid. The cocktail contains 8 inhibitors targeting against the major classes of proteases as outlined in the following table
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Products
Target?
Products
Target Proteases
AEBSF, HCl
Serine Proteases
Leupeptin
Cysteine Proteases and Trypsin-like Proteases
Aprotinin
Broad Spectrum, Serine Proteases
Pepstatin A
Aspartic Proteases
Bestatin
Aminopeptidase B and Leucine Aminopeptidase
Phosphoramidon
Metalloproteases
Metalloendopeptidases
E-64
Cysteine Proteases
Antipain
Papain, Cathepsin A/B
Recommended Usage: This cocktail has been optimized for effectiveness, convenience, stability, reproducibility and has been tested for general use. It inhibits activity in extracts from almost any tissue or cell types including animals, plants, yeasts, bacteria and fungi.?
Reconstitute each vial with 1 ml of water to obtain a concentrated stock solution. Dilute this conc stock solution to 1X before use. Recommended dilution is 1:100 (based on the application). One vial is recommended for 10g-20g of tissue or cell extract.
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Additional Information
Product #
T-2493
Appearance
White Lyophilized Solid. Hygroscopic
Purity
Suitable for use as Protease Inhibitors
Solubility
in water, aqueous buffers or add directly to extraction media