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Details

Host:?Mouse

Isotype:?IgG

Class:?Monoclonal

Immunogen:?Synthetic peptide corresponding to DYKDDDDK (FLAG)

Applications:

Red-colored affinity matrix consists of affinity purified Anti-DYKDDDDK (FLAG) Tag (FG4R) monoclonal antibody covalently attached to red beaded agarose (Separopore?). Suitable for purifying N-terminal, Met-N-terminal, C-terminal DYKDDDDK (FLAG) fusion proteins, 3x DYKDDDDK (FLAG) fusion proteins; greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-Flag Red-Separopore? 4B-CL is reliable tool?for purifying N-terminal, Met-N-terminal, C-terminal DYKDDDDK (FLAG) fusion proteins, 3x DYKDDDDK (FLAG) fusion proteins.

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Technical Specifications:

• Matrix: Separopore? 4B-CL (crosslinked agarose beads, 4%)
• Concentration:?25 mg Red Separopore ? per mL of buffer
• Particle size range: 52 – 165 μm?
• Ligand coupling method: CNBr activation?
• Binding capacity: approximately ~600 μg/ml drained gel?
• Exclusion limit: approximately 2 x 10⁷
• pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)?
• Supplied as 50% suspension in PBS, containing 50% glycerol.?

Additional Information

Additional Information