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Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM181; H3K79me2, Histone H3 (dimethyl K79)
品牌：RevMAb
貨號：31-1065-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [RM181]
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79)
Catalog No. 31-1065-00
Clone Name RM181
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM181
Specificity RM181 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). ). Very slightly cross-reacts with monomethylated Lysine 79 (K79me1) at high concentration. No cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.
Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79).
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.25 ug/mL – 1 ug/mL;
IHC: 0.1 ug/mL – 1 ug/mL;
ChIP: 2 ug/mL- 10 ug.mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
.
Product Images
RM181

RM181 specifically reacts to Histone H3 dimethylated
 at Lysine 79 (K79me2). Very slightly cross reactivity
 with monomethylated Lysine 14 (K14me1), and no
 cross reactivity with non-modified Lysine 79 (K79 ctrl),
 trimethylated Lysine 79 (K79me3), or other methylations
 in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
 and acid extracts of HeLa cells (2), using RM181
 at 0.25 ug/mL, showed a band of histone H3
dimethylated at Lysine 79 (K79me2) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K79me2 antibody
 (RM181, 5ug). Real-time PCR was performed using primers
specific to the gene indicated.

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
Anti-Dimethyl-Histone H3 (Lys79) antibody, RM181.


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