RM162 specifically reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p). No cross reactivity with non-modified Lysine 9/ Serine 10, methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or phosphorylation at Serine 9 ONLY in Histone H3.
Western Blot of acid extracts of HeLa cells (1) and recombinant histone H3.3 (2), using RM162 at 0. 01 ug/mL, showed a band of histone H3 modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p) in HeLa cells.
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