RM253 reacts to human and mouse alpha smooth muscle Actin (alpha-Actin-2). This antibody may also react to bovine or rat alpha smooth muscle Actin, as predicted by immunogen homology
Immunogen
A synthetic peptide corresponding to the N-terminus of human alpha smooth muscle Actin
Application
Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity
Human, Mouse
(Rat, Bovine)
Conjugate
None
Intended Use
Research Use Only
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Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size
100 uL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
Clone Type
Monoclonal
Isotype
Rabbit IgG
Usage
Immunohistochemistry (IHC): 1:1000-1:2500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Keywords
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Product Images
Immunohistochemical staining of formalin fixed and paraffin embedded human heart tissue sections using Anti-alpha smooth muscle Actin RM253 at a 1:2500 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue sections using Anti-alpha smooth muscle Actin RM253 at a 1:2500 dilution.
Western Blot of mouse heart tissue lysate, using Anti-alpha smooth muscle Actin RM253 at a 1:1000 dilution.