Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM195
Specificity
RM195 reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
Immunogen
A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3).
Application
Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity
Human
Vertebrates
Conjugate
None
Intended Use
Research Use Only
.
Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size
100 ug
Concentration
1.0 mg/mL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
RM195 specifically reacts to Histone H4 mono- methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a) or symmetric dimethylated Arg 3 (R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM195 at 0.5 ug/mL, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody (clone RM195) (red). Actin filaments have been labeled with fluorescein phalloidin (green).