Rabbit monoclonal to Phospho-Stat3 (Tyr705), STAT3 (phospho Tyr705)
Catalog No.
31-1142-00
Clone Name
RM261
Product Data Sheet
Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody Data Sheet RM261
Specificity
RM261 reacts to human Stat3 only when phosphorylated at Tyr705. There is no cross-reactivity to stat3 without phosphorylation at Tyr705. This antibody may also react to mouse or rat Phospho-Stat3 (Tyr705), as predicted by immunogen homology.
Immunogen
A phospho-peptide corresponding to human Phospho- Stat3 (Tyr705)
Application
Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity
Human
(Mouse, Rat)
Conjugate
None
Intended Use
Research Use Only
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Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size
100 uL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
Clone Type
Monoclonal
Isotype
Rabbit IgG
Usage
Immunohistochemistry (IHC): 1:1000-1:10,000 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Keywords
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Product Images
Western Blot of A431 cell lysates, nontreated (-) or treated (+) with EGF, using Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody (clone RM261) at a 1:1000 dilution, showed Phospho-Stat3 (Tyr705) in EGF-treated A431 cells.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody (clone RM261) at a 1:10,000 dilution.